Molecular Analyses of DNA repair
I am a microbiologist whose main areas of scientific expertise are in bacteriology & molecular biology. I enjoy conducting research in the regulation & evolution of bacterial pathogenicity, and the genetic responses of bacteria to environmental stimuli. In my lab, we use various microbiology, molecular biology and biochemistry techniques to examine the response of a model soil bacterium, Acinetobacter baylyi strain ADP1, to DNA damage. One project involves the UmuD protein, which typically functions in the SOS response pathway. In ADP1, this protein is larger than other UmuD homologs, is regulated differently than expected, and has novel functions, and we’re investigating how it does what it does. Another current project involves studying the other members of the genus Acinetobacter for their properties of DNA damage responses.
•J.M. Hare, J. A. Bradley*, T. J. Elam*, and Ching-li Lin*. 2012. Diverse DNA damage responses in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii. Microbiology 158: 601-611.
•J.M. Hare, S. Adhikari*, K. V. Lambert*, A. E. Hare and A. N Grice. 2012. The Acinetobacter Regulatory UmuDAb Protein Cleaves in Response to DNA Damage with Chimeric LexA/UmuD Characteristics. FEMS Microbiology Letters 334: 57-65.
•J. M. Hare, S. N. Perkins, and L. A. Gregg-Jolly. 2006. A constitutively expressed, truncated umuDC regulates the recA-dependent DNA damage-induction of a gene in Acinetobacter baylyi strain ADP1. Applied and Environmental Microbiology 72:4036-4043.
•I. Zwir, D. Shin, A. Kato, K. Nishino, T. Latifi, F. Solomon, J. M. Hare, H. Huang and E. A. Groisman. 2005. Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Proceedings of the National Academy of Sciences 102:2862-2867.
•Hare, J.M. and K.A. McDonough. 1999. High-Frequency RecA-Dependent and –Independent Mechanisms of Congo Red Binding Mutations in Yersinia pestis. Journal of Bacteriology 181:4896-4904.
•Hare, J.M., A.K. Wagner, and K.A. McDonough. 1999. Independent Acquisition and Insertion into Different Chromosomal Locations of the Same Pathogenicity Island in Yersinia pestis and Yersinia pseudotuberculosis. Molecular Microbiology 31:291-303.
•McDonough, K.A., and J.M. Hare. 1997. Homology with a Repeated Yersinia pestis DNA Sequence IS100 Correlates with Pesticin Sensitivity in Yersinia pseudotuberculosis. Journal of Bacteriology 179:2081-2085.
•Kemp, J.D., K.S. Smith, J.M. Mayer, F. Gomez, J.A. Thorsen, P.W. Naumann. 1992. Effects of Anti-Transferrin Receptor Antibodies on the Growth of Neoplastic Cells. Pathobiology 60:27-32.